01510nas a2200157   4500008004100000245010200041210006900143260000800212300001100220490000800231520101500239100001401254700001701268700001801285856004901303       1996                        eng d00aMolecular genetic analysis of sperm competition in the damselfly Ischnura elegans (Vander Linden)0 aMolecular genetic analysis of sperm competition in the damselfly  cOct  a1343-90 v2633 a{Sperm competition can be a powerful selective force in the evolution of mating systems. Several odonate species have attracted study to assess the extent and mechanism of last-male sperm precedence. Members of the genus Ischnura (Zygoptera) display a particularly interesting range of mating systems, and Ischnura elegans was selected for study. Polymorphic microsatellites were cloned, sequenced and used to determine paternity of I. elegans larvae, to reveal patterns of sperm precedence. More than 3000 larvae, collected from both wild and captively bred I. elegans females, were typed for one or two microsatellite loci and paternity was determined by comparison with parental genotypes. Microsatellite typing showed that most wild-caught females had mated with several males. Analysis of offspring from females which mated in captivity showed that multiple-matings result in a large proportion of last-male sperm precedence (mean value for immediate last male precedence is 0.79 +/- 0.2 (+/- s.d.; n = 111 aCooper, G1 aMiller, P, L1 aHolland, P, W  uhttps://worldodonata.myspecies.info/node/276